3D ultrastructural organization of whole Chlamydomonas reinhardtii cells studied by nanoscale soft x-ray tomography

The complex architecture of their structural elements and compartments is a hallmark of eukaryotic cells. The creation of high resolution models of whole cells has been limited by the relatively low resolution of conventional light microscopes and the requirement for ultrathin sections in transmission electron microscopy. We used soft x-ray tomography to study the 3D ultrastructural organization of whole cells of the unicellular green alga Chlamydomonas reinhardtii at unprecedented spatial resolution. Intact frozen hydrated cells were imaged using the natural x-ray absorption contrast of the sample without any staining. We applied different fiducial-based and fiducial-less alignment procedures for the 3D reconstructions. The reconstructed 3D volumes of the cells show features down to 30 nm in size. The whole cell tomograms reveal ultrastructural details such as nuclear envelope membranes, thylakoids, basal apparatus, and flagellar microtubule doublets. In addition, the x-ray tomograms provide quantitative data from the cell architecture. Therefore, nanoscale soft x-ray tomography is a new valuable tool for numerous qualitative and quantitative applications in plant cell biology

Scaling of loop-erased walks in 2 to 4 dimensions

We simulate loop-erased random walks on simple (hyper-)cubic lattices of dimensions 2,3, and 4. These simulations were mainly motivated to test recent two loop renormalization group predictions for logarithmic corrections in $d=4$, simulations in lower dimensions were done for completeness and in order to test the algorithm. In $d=2$, we verify with high precision the prediction $D=5/4$, where the number of steps $n$ after erasure scales with the number $N$ of steps before erasure as $n\sim N^{D/2}$. In $d=3$ we again find a power law, but with an exponent different from the one found in the most precise previous simulations: $D = 1.6236\pm 0.0004$. Finally, we see clear deviations from the naive scaling $n\sim N$ in $d=4$. While they agree only qualitatively with the leading logarithmic corrections predicted by several authors, their agreement with the two-loop prediction is nearly perfect.Comment: 3 pages, including 3 figure

Difference system for Selberg correlation integrals

The Selberg correlation integrals are averages of the products $\prod_{s=1}^m\prod_{l=1}^n (x_s - z_l)^{\mu_s}$ with respect to the Selberg density. Our interest is in the case $m=1$, $\mu_1 = \mu$, when this corresponds to the $\mu$-th moment of the corresponding characteristic polynomial. We give the explicit form of a $(n+1) \times (n+1)$ matrix linear difference system in the variable $\mu$ which determines the average, and we give the Gauss decomposition of the corresponding $(n+1) \times (n+1)$ matrix. For $\mu$ a positive integer the difference system can be used to efficiently compute the power series defined by this average.Comment: 21 page

‘Super disabilities’ vs ‘Disabilities’?:Theorizing the role of ableism in (mis)representational mythology of disability in the marketplace

People with disabilities (PWD) constitute one of the largest minority groups with one in five people worldwide having a disability. While recognition and inclusion of this group in the marketplace has seen improvement, the effects of (mis)representation of PWD in shaping the discourse on fostering marketplace inclusion of socially marginalized consumers remain little understood. Although effects of misrepresentation (e.g., idealized, exoticized or selective representation) on inclusion/exclusion perceptions and cognitions has received attention in the context of ethnic/racial groups, the world of disability has been largely neglected. By extending the theory of ableism into the context of PWD representation and applying it to the analysis of the We’re the Superhumans advertisement developed for the Rio 2016 Paralympic Games, this paper examines the relationship between the (mis)representation and the inclusion/exclusion discourse. By uncovering that PWD misrepresentations can partially mask and/or redress the root causes of exclusion experienced by PWD in their lived realities, it contributes to the research agenda on the transformative role of consumption cultures perpetuating harmful, exclusionary social perceptions of marginalized groups versus contributing to advancement of their inclusion

Vicious Random Walkers and a Discretization of Gaussian Random Matrix Ensembles

The vicious random walker problem on a one dimensional lattice is considered. Many walkers take simultaneous steps on the lattice and the configurations in which two of them arrive at the same site are prohibited. It is known that the probability distribution of N walkers after M steps can be written in a determinant form. Using an integration technique borrowed from the theory of random matrices, we show that arbitrary k-th order correlation functions of the walkers can be expressed as quaternion determinants whose elements are compactly expressed in terms of symmetric Hahn polynomials.Comment: LaTeX, 15 pages, 1 figure, minor corrections made before publication in Nucl. Phys.

Determination of the exponent gamma for SAWs on the two-dimensional Manhattan lattice

We present a high-statistics Monte Carlo determination of the exponent gamma for self-avoiding walks on a Manhattan lattice in two dimensions. A conservative estimate is \gamma \gtapprox 1.3425(3), in agreement with the universal value 43/32 on regular lattices, but in conflict with predictions from conformal field theory and with a recent estimate from exact enumerations. We find strong corrections to scaling that seem to indicate the presence of a non-analytic exponent Delta < 1. If we assume Delta = 11/16 we find gamma = 1.3436(3), where the error is purely statistical.Comment: 24 pages, LaTeX2e, 4 figure

Diagnostic potential of plasma carboxymethyllysine and carboxyethyllysine in multiple sclerosis

<p>Abstract</p> <p>Background</p> <p>This study compared the level of advanced glycation end products (AGEs), <it>N</it>-(Carboxymethyl)lysine (CML) and <it>N</it>-(Carboxyethyl)lysine (CEL), in patients with multiple sclerosis (MS) and healthy controls (HCs), correlating these markers with clinical indicators of MS disease severity.</p> <p>Methods</p> <p>CML and CEL plasma levels were analyzed in 99 MS patients and 43 HCs by tandem mass spectrometry (LC/MS/MS). Patients were stratified based on drug modifying therapies (DMTs) including interferon beta, glatiramer acetate and natalizumab.</p> <p>Results</p> <p>The level of plasma CEL, but not CML, was significantly higher in DMT-naïve MS patients when compared to HCs (P < 0.001). Among MS patients, 91% had higher than mean plasma CEL observed in HCs. DMTs reduced CML and CEL plasma levels by approximately 13% and 40% respectively. CML and CEL plasma levels correlated with the rate of MS clinical relapse.</p> <p>Conclusion</p> <p>Our results suggest that AGEs in general and CEL in particular could be useful biomarkers in MS clinical practice. Longitudinal studies are warranted to determine any causal relationship between changes in plasma level of AGEs and MS disease pathology. These studies will pave the way for use of AGE inhibitors and AGE-breaking agents as new therapeutic modalities in MS.</p

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Multifunctional composite nanoprobes consisting of iron oxide nanoparticles linked to silver and gold nanoparticles, Ag–Magnetite and Au–Magnetite, respectively, were introduced by endocytic uptake into cultured fibroblast cells. The cells containing the non-toxic nanoprobes were shown to be displaceable in an external magnetic field and can be manipulated in microfluidic channels. The distribution of the composite nanostructures that are contained in the endosomal system is discussed on the basis of surface-enhanced Raman scattering (SERS) mapping, quantitative laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping, and cryo soft X-ray tomography (cryo soft-XRT). Cryo soft-XRT of intact, vitrified cells reveals that the composite nanoprobes form intra-endosomal aggregates. The nanoprobes provide SERS signals from the biomolecular composition of their surface in the endosomal environment. The SERS data indicate the high stability of the nanoprobes and of their plasmonic properties in the harsh environment of endosomes and lysosomes. The spectra point at the molecular composition at the surface of the Ag–Magnetite and Au–Magnetite nanostructures that is very similar to that of other composite structures, but different from the composition of pure silver and gold SERS nanoprobes used for intracellular investigations. As shown by the LA-ICP-MS data, the uptake efficiency of the magnetite composites is approximately two to three times higher than that of the pure gold and silver nanoparticles.Peer Reviewe